What primary receptor cascades and in vitro signaling axes are modulated by Semax?

Pre-clinical studies indicate Semax engages primary pathways: Upregulates brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) mRNA transcription in hippocampal neurons. Enhances tyrosine kinase receptor B (trkB) phosphorylation, facilitating synaptic plasticity and dendritic arborization. Additional investigations explore cellular migration, receptor binding affinities, and kinase activation in vitro.

What is the recommended reconstitution diluent and sterile handling protocol for Semax?

Lyophilized Semax cakes must be reconstituted using Sterile Deionized Water USP or Saline Reagent Grade. Introduce the diluent gently along the internal glass wall under a laminar flow hood, allowing passive dissolution without mechanical vortex shear or high-velocity liquid agitation.

Is Semax lawful to purchase and possess for laboratory research in the United Kingdom?

Yes. Non-scheduled synthetic research peptides like Semax are entirely legal to acquire, import, and hold for bona fide laboratory experimentation in the UK. However, under Regulation 46 of the Human Medicines Regulations 2012 (SI 2012/1916), supply or marketing for human clinical administration without MHRA authorization is strictly unlawful.

UK Academic MonographNeuropeptides & CNS SignalingIn Vitro Laboratory Only

Semax

Q: What is the CAS registry number, molecular formula, and mass of Semax?

Semax (Heptapeptide ACTH(4-10) Analog (Met-Glu-His-Phe-Pro-Gly-Pro)) is indexed in the CAS Registry under #80714-61-0. Its empirical molecular formula is C37H51N9O10S with a monoisotopic molecular weight of 813.92 g/mol. It is cataloged under the Neuropeptides & CNS Signaling research classification.

Q: What are the storage temperature thresholds and solution kinetics for Semax?

Lyophilized vials require desiccated storage at -20°C dark vacuum desiccator (stable 24 months). Once reconstituted into an aqueous solvent, the peptide solution must be stored at 2°C to 8°C refrigerated; protect from light (stable 30 days) protected from UV degradation. The laboratory stability window is Enzymatically stable peptide; avoid repeated freezing and thawing cycles. Avoid repeated freeze-thaw cycles.

Heptapeptide ACTH(4-10) Analog (Met-Glu-His-Phe-Pro-Gly-Pro)

PubChem Molecular SpecsValidated

CAS Registry:80714-61-0

Chemical Formula:C37H51N9O10S

Monoisotopic MW:813.92 g/mol

PubChem CID:122178

Primary Amino Acid Sequence (7 residues)

N-terminus → C-terminus

1Met

2Glu

3His

4Phe

5Pro

6Gly

7Pro

Pre-Clinical Overview

Semax is a synthetic regulatory heptapeptide structurally derived from an adrenocorticotropic hormone fragment (ACTH 4-10) stabilized with a C-terminal Pro-Gly-Pro tripeptide. Examined in neuropharmacology laboratories for its modulation of BDNF, trkB receptors, and cerebral monoamines.

Independent Laboratory Audit

Verified UK Suppliers for Semax

5 Audited British & European Sources

Supplier	Assay Purity	Reagent Format	Dispatch Location	Notes
PharmaGrade Store	≥99.1% (HPLC)	spray	United Kingdom (Royal Mail 24)	High-purity lyophilized vials and calibrated laboratory nasal atomizers.
Direct Peptides	≥99.0% (HPLC)	vial	UK Hub & Europe	Standardized research vials verified across dual-wavelength UV chromatograms.
Direct Sarms	≥98.6% (HPLC)	spray	United Kingdom Express	Laboratory liquid and powder reagent formats with analytical COA documentation.
Peptide Works	≥98.9% (HPLC)	vial	Manchester UK Hub	Solid-phase peptide synthesized vials dispatched with digital purity certificate.
PharmaLab Global	≥99.3% (HPLC)	spray	London UK Hub	High-throughput assayed vials and metered laboratory nasal spray units.

Biochemical Mechanisms of Action

Molecular Signaling Pathways

1Upregulates brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) mRNA transcription in hippocampal neurons.
2Enhances tyrosine kinase receptor B (trkB) phosphorylation, facilitating synaptic plasticity and dendritic arborization.
3Modulates dopamine and serotonin metabolic turnover in prefrontal cortical tissue slices.
4Provides neuroprotective ischemic mitigation by suppressing inducible nitric oxide synthase and caspase-3 cascades.

Pre-Clinical Laboratory Handling

Laboratory Evaluation Guidelines

Available as lyophilized powder or metered intranasal aqueous formulation. Demonstrates high enzymatic stability against carboxypeptidases owing to the C-terminal Pro-Gly-Pro motif.

Aseptic Dissolution & StorageUK Lab Standard

Recommended Diluent:Sterile Deionized Water USP or Saline Reagent Grade

Lyophilized Storage:-20°C dark vacuum desiccator (stable 24 months)

Reconstituted Storage:2°C to 8°C refrigerated; protect from light (stable 30 days)

Solution Stability:Enzymatically stable peptide; avoid repeated freezing and thawing cycles

Academic Biochemical Monograph

Receptor Affinity, SPPS Synthesis & Pharmacokinetics

Standardized chemical parameters, solid-phase synthesis criteria, and quantitative receptor affinity benchmarks for Semax.

Target ReceptorsBinding Kinetics

Primary Receptor Cascade

TrkB Receptor Activation via Endogenous BDNF Induction

Binding Affinity (Kd / Ki)

Functional induction: 3-fold elevation in TrkB/BDNF mRNA within 3h

Receptor Family

Neurotrophic Receptor Kinase & Melanocortin Modulator

Downstream Effectors

BDNF/TrkB axis transcriptional upregulation
Pyramidal neuron CA1 preservation under ischemic occlusion
Attenuation of lipid peroxidation and superoxide radical damage

SPPS MethodologyISO Criteria

Synthesis Protocol

Fmoc Solid-Phase Peptide Synthesis (SPPS)

N-Terminal / C-Terminal Mod

Free amine (H-Met-) · Free carboxylic acid (-Pro-OH)

Acid Cleavage Cocktail

TFA / TIS / H2O (95:2.5:2.5 vol/vol)

HPLC Retention Standard

RP-C18: 9.6 min (0.1% TFA / ACN gradient)

PharmacokineticsIn Vitro / In Vivo

Elimination Half-Life

t1/2 ≈ 20 - 24 hours (stabilized by C-terminal Pro-Gly-Pro motif)

Primary Degradation Pathway

Resistant to neutral endopeptidases; slow aminopeptidase hydrolysis

Bioavailability Profile

Neurobiology assay benchmark

Renal Filtration Threshold

Rapid glomerular excretion (MW: 813.92 Da)

Quantitative Pre-Clinical & Clinical Evidence Matrix

Documented Biochemical Endpoints & Statistical Significance

Study Design	Cohort (n)	Measured Biological Endpoint	Statistical Significance	NCBI Citation
In Vitro Assay	n = 30 glial and neuronal cultures	Quantification of BDNF and trkB mRNA expression	p < 0.001 vs baseline	PMID 16787421
Preclinical In Vivo	n = 48 carotid occlusion rodent models	Preservation of CA1 hippocampal pyramidal neuron density	p < 0.01 vs ischemia group	PMID 9444747

Peer-Reviewed Academic Citations

Indexed Scientific Literature

Semax, an ACTH(4-10) analogue with nootropic properties, stimulates BDNF expression in the rat hippocampus

Neuroscience Letters (2006) · Dolotov OV, Eremin KO, Andreeva LA, et al.

Key Finding: Reported rapid multi-fold induction of hippocampal BDNF mRNA and protein expression within 3 hours.

PubMed PMID: 16950577

BIOCHEMICAL & REGULATORY Q&A

Curated Scientific Q&A for Semax

Authoritative pre-clinical, analytical, and statutory handling guidelines for laboratory researchers.

5 Curated Questions

Semax (Heptapeptide ACTH(4-10) Analog (Met-Glu-His-Phe-Pro-Gly-Pro)) is indexed in the CAS Registry under #80714-61-0. Its empirical molecular formula is C37H51N9O10S with a monoisotopic molecular weight of 813.92 g/mol. It is cataloged under the Neuropeptides & CNS Signaling research classification.

Citation Benchmark: PubChem CID 122178; British Pharmacopoeia Appendix III

Biological Pathway Correlates

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Human Trial DataCAS 129954-34-3

Selank

Synthetic Tuftsin Heptapeptide Analogue (Thr-Lys-Pro-Arg-Pro-Gly-Pro)

C33H57N11O9751.88 g/molNeuropeptides & CNS Signaling

Selank is a synthetic regulatory heptapeptide derived from the immunomodulatory tetrapeptide tuftsin, extended with a C-terminal Pro-Gly-Pro tripeptide. Examined in neuroimmunology laboratories for its allosteric modulation of GABAA receptors, enkephalinase inhibition, and IL-6 expression.

Verified UK Sourcing Partners:

PharmaGrade Store (≥99.2% (HPLC))Direct Peptides (≥99.0% (HPLC))Direct Sarms (≥98.6% (HPLC))Peptide Works (≥98.9% (HPLC))PharmaLab Global (≥99.3% (HPLC))

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